Coding

Part:BBa_K1727003:Design

Designed by: Ying Kuan   Group: iGEM15_TCU_Taiwan   (2015-09-05)


Epinecidin-1 with signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 304
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 247


Design Notes

We treated signal peptide upstream of the N-terminal of Epinecidin-1 to facilitate the peptide production. To make sure the secretion system is work we had modified Epinecidin-1 amino acid sequence by attached an Ala at the N-terminal of it .

We use PQE 60 as vector,so we design BamH I enzyme site at 5' end and Bgl II enzyme at 3' end.

Source

Epinecidin-1 is the genomic sequence for the skin mucus of Epinephelus coioides. We got this sequence from Academia Sinica. Signal peptide is artificial synthesis.

References

Peng, K.C., et al., Using an improved Tol2 transposon system to produce transgenic zebrafish with epinecidin-1 which enhanced resistance to bacterial infection. Fish Shellfish Immunol, 2010. 28(5-6): p. 905-17.

Fujii, T. and K. Miyashita, Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans. J Gen Microbiol, 1993. 139(4): p. 677-86.